difco manual microbiological media

difco manual microbiological media

Difco Laboratories, Detroit. The information you enter here will be stored in the downloaded file to assist you in managing your downloaded PDFs locally. Please wait for an email containing a link to download the PDF. Help keep BHL free and open! Please try again and if the problem persists, please send us feedback. To browse Academia.edu and the wider internet faster and more securely, please take a few seconds to upgrade your browser. Related Papers DIFCO A BBL MANUAL By Ershad Mohibi 211124 By Arely Montesinos Castillo Microbiology Manual By LASINRANG ADITIA, S.Si Bacteriological Analytical Manual Online By Sannie Machado Bacteriological Analytical Manual By Heo Ng?c READ PAPER Download pdf. Some features of WorldCat will not be available.By continuing to use the site, you are agreeing to OCLC’s placement of cookies on your device. Find out more here. Numerous and frequently-updated resource results are available from this WorldCat.org search. OCLC’s WebJunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus issues in their communities.However, formatting rules can vary widely between applications and fields of interest or study. The specific requirements or preferences of your reviewing publisher, classroom teacher, institution or organization should be applied. Please enter recipient e-mail address(es). Please re-enter recipient e-mail address(es). Please enter your name. Please enter the subject. Please enter the message. Publisher: Sparks, MD: Difco Laboratories, Division of Becton Dickinson and Co., 2003.Please select Ok if you would like to proceed with this request anyway. All rights reserved. You can easily create a free account. The 13-digit and 10-digit formats both work. Please try again.Please try again.Please try again. Used: GoodCover art may differ from stock photo.Then you can start reading Kindle books on your smartphone, tablet, or computer - no Kindle device required.http://elrey-uslugi.ru/media/bosch-tankless-water-heater-aq-250-sx-ng-manual.xml

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Register a free business account To calculate the overall star rating and percentage breakdown by star, we don’t use a simple average. Instead, our system considers things like how recent a review is and if the reviewer bought the item on Amazon. It also analyzes reviews to verify trustworthiness. Please try again later. Dr. C. 5.0 out of 5 stars. Used: GoodPages are clean and unmarked. Page edges do have spots of yellowing. Very minor edgewear and corner bumps. Tulsa's best Used Bookstore. Located on South Mingo Road since 1991. No-hassle return policy if not completely satisfied.Please try again.Please try again.Please try again. This edition has been completely revised and rewritten. The purpose of the Manual is to provide information about products used in microbiology. Then you can start reading Kindle books on your smartphone, tablet, or computer - no Kindle device required. Register a free business account To calculate the overall star rating and percentage breakdown by star, we don’t use a simple average. Book Acceptable. No dust jacket. Cover soiled. Owner's name on front endpage.We have over 1 Million books for sale on our website and another 1 Million books for sale in our 3 locations. Items on Abe are not necessarily in stock at our retail locations. Please do not call the stores to place orders for Abe Books sales. We have a very active online inventory and as such, we can receive multiple orders for the same item. We fill those orders on a first come first serve basis, but will refund promptly any items that are out of stock. Since 1980 it has always been about the books. ALL kinds of books from 95 cent children's paperbacks to five figure rare and collectibles. A merging of the old and new is where we started, and it is where we are today. Members ABAA, ILAB.They vouch for the authenticity of all items offered for sale.Our general description is conservative and the book should be as good or better than our general description may indicate.http://0377cw.com/upload/15985832081503464754.xml

RETURNS are cheerfully accepted up to 30 days. We ship out within 1-2 business days and U.S. Standard Shipments usually arrive within 6-9 business days, Priority 3-6. We want you to be satisfied with the book you order. Our general description is conservative and the book should be as good or better than our general descr. More Information Heavier books may require additional shipping. Faster Needs Please Inquire. All Rights Reserved. Put multi-word tags in quotation marks.Sparks, MD: Difco Laboratories, Division Of Becton Dickinson And Co., 2003. Print. Please use this display as a guideline and modify as needed. Use quotes for multi-word tags. Specifications Book Details Imprint Andesite Press Dimensions Width 19 mm Height 234 mm Length 156 mm Weight 499 gr Read More Have doubts regarding this product. Post your question Safe and Secure Payments. Easy returns. 100% Authentic products. DIFCO and BBL are registered trademarksFLOW CHARTS: Outlines theThe CD includes over 400Discount cannot be applied to the price of the Difco Manual. Inc PO Box 1130 Lawrence, Kansas 66044. Please try again.Tuesday, Sep 22No customer signatures are required at the time of delivery. To pay by cash, place cash on top of the delivery box and step back. Order delivery tracking to your doorstep is available.This work was reproduced from the original artifact, and remains as true to the original work as possible. Therefore, you will see the original copyright references, library stamps (as most of these works have been housed in our most important libraries around the world), and other notations in the work. This work is in the public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity (individual or corporate) has a copyright on the body of the work. As a reproduction of a historical artifact, this work may contain missing or blurred pages, poor pictures, errant marks, etc.

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Join plum PLUS This work is in the public domain in the United States of America, and possibly other nations. To ensure a quality reading experience, this work has been proofread and republished using a format that seamlessly blends the original graphical elements with text in an easy-to-read typeface. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant. NO, I do not recommend this product. Your review has been submitted and will appear here shortly. This book may have occasional imperfections such as missing or blurred pages, poor pictures, errant marks, etc.We believe this work is culturally This book may have occasional imperfections such as missing or blurred pages, poor pictures, errant marks, etc.We believe this work is culturally important, and despite the imperfections, have elected to bring it back into print as part of our continuing commitment to the preservation of printed works worldwide. MICROBIOLOGICAL and CLINICAL Printed in U. S. A. Acknowledgment is also made of the use of Foreword. This edition of the Difco Manual, the ninth published since 1927, has The Ninth Edition is the first to When we first introduced prepared dehydrated culture media to the Today, Difco Difco products are not only being used by a continually increasing number Research laboratories maintained by the Federal Government are also Recommendation and approval have been extended to our products by American Public Health Association, the American Dairy Science Associa- Bacto Dehydrated Culture Media and laboratory reagents are prepared Each ingredient is The new section The dehydrated media are stable and resist deterioration over long Culture Media are truly economical when actual cost comparisons are Grateful acknowledgment is made of the support we have received It is the desire of our organization to Difco Laboratories. Table of Contents Introduction 11.

Origin of Dehydrated Culture Media 15. General Conditions Pertaining to the Cultivation of Microorganisms.. 16. Preparation of Media from Dehydrated Culture Media, Difco. 21. Dehydrated Culture Media 23. Guides for the Selection of Culture Media 23. Media for Examination of Water and Sewage 29. Supplementary Media for Water and Sewage Examination. 41. Media for Examination of Dairy and Other Food Products. 57. Supplementary Media for Dairy and Other Food Products. 68. Media for Lactobacilli 72. Media for Cultivation of Pathogenic Microorganisms 76. Infusion Media 76. Peptone Media (without infusions) 99. Differential Media 130. Schema for Examination of Stools 130. Primary Plating Media 131. Differential Liquid Enrichments 156. Differential Tube Media 159. Sterility Test Media 195. Media for Microbiological Assays 203. Media for the Assay of Antibiotics 203. Media for the Assay of Vitamins and Amino Acids 212. Media for Mycology 237. Ingredients of Culture Media 255. Peptones, Difco 255. Enzymatic Hydrolysates 255. Hydrolysates, Acid 265. Amino Acids 268. Extracts 269. Enrichments 271. Enzymes 278. Bile Products 286. Dehydrated Meats for Infusions 288. Solidifying Agents 290. Carbohydrates, Polyhydric Alcohols and Glucosides 291. Carbohydrate Solutions in Ampuls 292 Biochemicals 296. Miscellaneous Ingredients 299. Tissue Culture Media Reagents, Difco 301. Methods of Tissue Culture 307. Serological Reagents for Diagnosis of Syphilis 309. Reagents for Complement-Fixation Tests 309. Reagents for Precipitation Tests 311. Cardiolipin Antigens 313. Reagents for Preparation of Antigens 315. Diagnostic Reagents 317. Miscellaneous Products 338. Dehydrated Media in Special Packages 338. Prepared Media in Tubes and Bottles 338. Index 343. Introduction. Bacteriology emerged as a definite branch of science as a result of the monu- When in 1876 Robert.

Koch, for the first time in history, propagated a pathogenic bacterium in pure The decade immedi- The fundamental principles elaborated at that time, of which the most im- Nevertheless, With a suitable culture The bacteriologist of The chemical analyses of bacteria indicate that they are essentially water plants, Almost without exception wherever bacteria occur in nature, and this is particu- So complex is the structure of many of these substances, Such alterations are effected by processes of hydrolysis, These changes are ascribed to the activities Abstract as such studies of bacterial metabolism may seem, their practical From these studies has come a better understanding Naegeli's report covered the use of a large variety of sub- The first reference to the use of peptone for the cultivation of microorganisms Because of its content of amino acids and other In the light of our present knowledge, proteins are believed to be complex com- When subjected to hydrolysis proteins yield metaproteins, proteoses, peptones, The intermediate products should be considered as classes of compounds, rather The relation of amino acids to bacterial metabolism, and the ability of bacteria Many other Indispensable as amino acids are to From the data thus far summarized, it is apparent that the problem of bacterial It is not improbable While the importance of nitrogenous substances for bacterial growth was It is highly desirable, in fact essential, to Many laboratory methods, such as The use of protein hydrolysates, particularly gelatin and casein, has stimu- The importance of these new paths of investigation Closely associated with research of this nature are such In this brief discussion of certain phases of bacterial nutrition we have at- Difco Laboratories has been en- Difco Dehydrated Culture Media, and Muenchen, 10:277:1880. Exp. Med., 13:365:1911. Infectious Diseases, 15:455:1914.

It is a pleasure to include, as a part of this book, the abstract given below It is to be noted that Dr. Frost's University of Wisconsin. Abstract of Paper at Boston (1909) Meeting of the Society of American Bacteri- In order to overcome the generally recognized faults of bacterial culture media, The author's work on this problem, covering nearly a decade of time, is con- There is apparently no reason why the different culture media cannot be put Not only the ordinary, but probably most of the It is interesting to note that Doerr, in Kraus and Uhlenhut: Handbuch der. Mikrobiologischen Technik, states he also prepared powdered culture media by The practical application of the dehydration of culture media was initiated and The development of microorganisms upon culture media is dependent upon a A satisfactory microbiological culture medium must contain available sources These were originally supplied in the form of the meat in- Beef extract frequently replaces meat infusions, but the preparation of this sub- The addition of peptone provides a readily available Peptone is used in culture media to supply an available form of nitrogen since Most organisms are capable Certain bacteria require additions of other food substances such as serum, Carbohydrates may also be desirable at times, and certain salts such as those of Dyes may be added to media as indicators of metabolic activity or because of their Growth promoting substances of a vitamin-like nature The consistency of a liquid medium may be modified by the addition of agar, One of the principal landmarks in bacteriology was the preparation of a Until the intro- Most bacteria are capable of growth under ordinary conditions of oxygen Between these Eh range.

Anaerobic conditions for growth of microorganisms are obtained in a number Methods of readily obtaining anaerobic conditions in the laboratory are dis- For anaerobic conditions a cotton plug is placed just A solution of The tube is then ready for Remove the percussion tip from the Seal the culture tube with a rubber stopper Proper moisture conditions must prevail in the culture media employed for the The pH or reaction of the culture medium, expressing its hydrogen ion concen- The pH or reaction of the It should be noted that additions of acid or alkali The usual range of temperature suitable for the growth of microorganisms lies All organisms exhibit three cardinal points in their thermic relations: In addition to a suitable temperature for growth of microorganisms it is Some organisms re- For example, media in plates inoculated with Incubators should have open containers filled with The media upon which microorganisms are grown must be sterile or free from The usual method for immediate sterilization of culture If larger volumes are to The medium is Tubes should be placed in racks or packed loosely in baskets. Flasks should never In the operation of the autoclave, all the enclosed air must be allowed to Pressure-temperature relations Pressure-Temperature Relations in Autoclave Pressure in Pounds Temperature Through the courtesy of. Dr. F. W. Tanner 2 we are able to reproduce the following chart which plainly Effect of Entrapped Air on Temperature of Autoclave. PER CENT AIR IN SItAM. When the operator is assured that all the air is replaced by steam, which is best When the ther-.

A maximum of 15 minutes is recommended for the sterilization of carbohydrate After the sterilization period Pressure should not drop too rapidly or the Pressure should, Ordinarily about 8, and not more than 1 2, minutes The media should be For the sterilization of coagulable material such as serum, see the method given Oversterilization or prolonged heating will change the composition of the The same lot of medium sterilized for This demonstrates that oversterilization resulted in a break- Agar media on prolonged sterilization or heating are apt to show a precipitate. Media contain- This flocculent agar Excessive heating of media also results in an increase in acidity. The reaction Some media which It is possible to destroy completely the jellifying properties Culture media which may be injured by autoclaving are sometimes sterilized This procedure consists of heating Body fluids and sera are sometimes sterilized External contamination of culture media is prevented by plugging the tubes or Plugs should fit neither too Tubes of Kligler Iron Agar The same medium with tubes loosely Media should always be stored in a cool moist atmosphere to prevent evapora- Agar tubes should be Blood or other body fluids to be cultured should always be taken prior to the If drugs have been administered their The addition of j!?-amino- Bacto-Penase, a concen- Bacto-Brain Heart. Infusion with P.A.B. and Agar with added Bacto-Penase is an ideal medium for The advantages of dehydrated media and their efficiency for the cultivation of a Public Health Association. The preparation of media from Bacto Dehydrated Culture Media is a time- The composition of each medium is stated on the label of the bottle with the For ease of preparation and for best results with Bacto. Dehydrated Media, a discussion of these methods is given in detail. Distilled water should be used in the rehydration of dehydrated culture media.

Distilled water that has been stored at room temperature for any length of It is recommended that freshly distilled water or boiled The quantity of dehydrated culture medium to use per liter is specified on each Best results will be obtained by adding the powder to A stirring rod may be used to The entire amount of distilled water is added when all Broth or liquid media are readily soluble in. Agar media must be heated to the boiling point for For small quanti- Another satisfactory The suspension is then finally added to the boiling Gelatin media are best Care should be exercised to avoid contamination of media during the rehydra- It has been shown that deter- Three highly Clean glassware For example, The importance of clean glassware in the assay Following rehydration, Difco culture media require no filtration. In some Levine E.M.B. Agar and Bacto-Niacin Assay Medium. Removal of these precipi- The filtration of any medium They were able to The addition of starch to the medium The same observation was also made with centri- Adjustment of the reaction of the medium is not required. The final reaction The temperature of the The ionization constant increases with a rise in Uniform standardized media are readily prepared in large or small amounts By the use of these products microbiolo- Dehydrated Culture Media The tables on the following pages will assist in the selection of media for vari- For the reason that media other than those listed may be preferred in some lab- Tests for Coliform Organisms. Plate Counts Presumptive Confirmed Completed. Nutrient Gelatin. Tryptone Glucose Extract. Agar. Lactose Broth. Lauryl Tryptose. Broth. Endo Agar. Levine E.M.B. Agar. Brilliant Green. Bile 2%. Formate Ricinoleate. Nutrient Agar. Control of Water Filtration Plant Operation. Selective Broths. Selective Agars. Differential Test Media. Fuchsin Lactose Broth. Brilliant Green Bile 2%. M.B.-B.C.P. Medium. Formate Ricinoleate Broth. Crystal Violet Broth. Eijkman Lactose Medium.

E C Medium. MacConkey Broth. MacConkey Agar. Violet Red Bile Agar. Desoxycholate Lactose. Brilliant Green Bile Agar. Bacto-Tryptone. M.R.-V.P. Medium. Koser Citrate Medium. Culture Media for the Examination of Dairy and Other Food Products. Plate Counts. Tryptone Glucose Extract Agar. Proteose Tryptone Agar. Beef Lactose Agar. Nutritive Caseinate Agar. Heart Infusion Agar. Brucella. Tryptose Agar. Lactobacilli. Tomato Juice Agar. Trypsin Digest Agar. Peptonized Milk. Skim Milk. Micro Assay Culture Agar. Micro Inoculum Broth. Snyder Test Agar. Hemolytic Streptococci. Coliform Organisms. Desoxycholate Agar. Desoxycholate Lactose Agar. Thermophiles. Dextrose Tryptone Agar. Thermoacidurans Agar. Molds and Yeasts. Potato Dextrose Agar. Malt Agar Phosphate. Starch Ag. Agar CO Ph 3 4J. S-r o'H. QCoffi 6 CO i G c3 Is Striated Jg C d cj Id M bo Proteose Peptone, No. 3, Difco. 10 g. Sodium Chloride 5 g. Bacto-Agar 15 g. Bacto-Veal Infusion Agar is prepared from select lean veal and is recom- It is also a suitable basal medium for enrichment by the addition of blood, To rehydrate the medium suspend 40 grams Bacto-Veal Infusion Agar in Since most organisms prefer a fresh medium with a moist surface, it is sug- One pound of Bacto-Veal Infusion Agar will make 11.3 liters of medium. Infusion from 50 g. Bacto- Peptone 10 g. Bacto-Agar 14 g. Tryptose and Bacto-Yeast Extract were satisfactory peptones in a medium for Bacto-PPLO Agar duplicates Bacto-PPLO Agar, enriched with Bacto-Ascitic Fluid or Bacto-PPLO Serum. Fraction will permit the development of colonies of PPLO visible microscopi- PPLO colonies are round with They vary from 10 to 500 microns in diameter (0.01-0.5 mm.) and grow into the Vacuoles are seen in the periphery of They are the large bodies characteristic of the pleuropneu- To rehydrate the medium, suspend 34 grams of Bacto-PPLO Agar in 1000 ml. Distribute in flasks and sterilize in the autoclave for 15 minutes at 15 pounds pres-.

Bacto-PPLO Serum Fraction or 25 per cent Bacto-Ascitic Fluid. Mix thoroughly The reaction of the unenriched medium will One pound of Bacto-PPLO Agar will make 13.3 liters unenriched medium. Beef Heart, Infusion from 250 g. Proteose Peptone, Difco 10 g. Bacto-Dextrose 2 g. Disodium Phosphate 2.5 g. Bacto-Brain Heart Infusion Agar is recommended as a solid medium for the A selective medium for The liquid medium, Bacto-Brain Heart Infusion, has for many years been This medium, solidified with agar to Heart Infusion with 2 per cent agar was more satisfactory than 1 per cent Dex- Incubation in an atmosphere of 5 per cent carbon dioxide was required for best HowelP used Bacto-Brain Heart Infusion to which was added 2 per cent Bacto-. Agar and 10 per cent sterile defibrinated horse blood for the cultivation of. Histoplasma capsulatum. A selective medium for the isolation of this organism Potato Dextrose Agar, the Brain Heart Infusion Agar gave a greater number of H. capsulatum from tissues of experimentally infected mice. Their results showed H. capsulatum from the tissues of the infected mice. Bacto-Brain Heart Infusion Agar contains 0.2 per cent Bacto-Dextrose, mak- Base or Bacto-Blood Agar Base, as discussed on pages 87, 115 and 88, should To rehydrate the medium, suspend 52 grams Bacto-Brain Heart Infusion Agar Heat to boiling to dissolve the medium com- A selective medium for fungi is prepared by adding 20 units penicillin and One pound of Bacto-Brain Heart Infusion Agar will make 8.7 liters of Edition:452: 1950. Proteose Peptone, Difco 10 g. Bacto-Dextrose 10 g. Bacto-Cystine Heart Agar, enriched with Bacto-Hemoglobin, is recommended The use of this medium is suggested Bacto-Cystine Heart Agar without enrichment sup- A large number of the media first em- Blood Dextrose Cystine Agar in his later investigation as being a satisfactory Cystine Heart Agar proved to be entirely satisfactory for the cultivation of. P. tularensis.

In three or four days the growth is sufficient for the preparation of Bacto-Cystine Heart Agar was originally developed in collaboration with. Rhamy. As mentioned in the paper by Rhamy, referred to above, W. M. Simp- Also cooperating in these preliminary trial Cystine Heart Agar and Bacto-Hemoglobin entirely satisfactory for growing. P. tularensis. When used with Bacto-Hemoglobin, the medium is prepared for use as A. Suspend 10.2 grams Bacto-Cystine Heart Agar in 100 ml.Sterilize in the. B. Place 2 grams Bacto-Hemoglobin in a dry flask and add 100 ml. cold The hemoglobin When a plain Cystine Dextrose Agar, without hemoglobin, is desired, the One pound of Bacto-Cystine Heart Agar will make 8.9 liters of the enriched, Bacto-Gasamino Acids, Technical.. 17.5 g. Starch 1.5 g. Bacto- Agar 1 7 g. Bacto-Mueller Hinton Medium duplicates the formula recommended by In an attempt to develop a simple transparent medium containing no heat- The medium chosen for study As a result of the fractionation In addition, they found Technical. Growth of the gonococcus and meningococcus on the developed For the cultivation of the gonococcus, it is imperative to have the incubation Satisfactory conditions can be obained if A can with a suitable About 200 ml. of water added in Plates incu- If the culture requires carbon dioxide for growth this To rehydrate the medium, suspend 38 grams of Bacto-Mueller Hinton. Medium in 1000 ml. of cold distilled water and heat to boiling to dissolve the It is recommended that the dissolved medium be distributed into test tubes One pound of Bacto-Mueller Hinton Medium is sufficient to prepare 11.9 Veal, Infusion from 375 g. Monosodium Phosphate 1.25 g. Sodium Chloride 3.75 g. Bacto-Brain Veal Agar is a medium containing extractives of fresh calf brain Bacto-Brain Veal Agar is prepared according to their Bacto-Brain Veal Agar is a satisfactory medium for the preparation of bacterial Brain Veal Agar for the isolation of the gonococcus.